Support method development in :

  1. ORF optimization and sequence, IRES, intron, RE analysis
  2. Optimization of mRNA secondary structure, signal sequence optimization
  3. Choice of promoter, Optimize promoter transcription start and Kozak , choice of plasmid, selection marker, and host
  4. Optimize pcd (picogram/cell/day) and volumetric productivity in broth via insert amplification
  5. Optimize recombinant induction protocol at lab and pilot scale, and optimize secretion efficiency
  6. Media optimization, pH, glutamine
  7. Clone stability
  8. Clone purity
  9. Glyco-isoform analysis