Development of recombinant MCB in E coli

Support method development in :

  1. ORF optimization and sequence, RE analysis
  2. Optimization of mRNA secondary structure
  3. Choice of promoter, Optimize promoter transcription start and Kozak, choice of plasmid, selection marker, and host
  4. Optimize pcd (picogram/cell/day) and volumetric productivity
  5. Optimize recombinant induction protocol at lab and pilot scale
  6. Media optimization
  7. Clone stability
  8. Clone purity

Development of recombinant MCB in CHO Cells:

Support method development in :

  1. ORF optimization and sequence, IRES, intron, RE analysis
  2. Optimization of mRNA secondary structure, signal sequence optimization
  3. Choice of promoter, Optimize promoter transcription start and Kozak , choice of plasmid, selection marker, and host
  4. Optimize pcd (picogram/cell/day) and volumetric productivity in broth via insert amplification
  5. Optimize recombinant induction protocol at lab and pilot scale, and optimize secretion efficiency
  6. Media optimization, pH, glutamine
  7. Clone stability
  8. Clone purity
  9. Glyco-isoform analysis

Tech Pack for E coli and CHO Expressed Products:

  1. Cell engineering
  2. Media engineering
  3. Operation optimization
  4. Bioreactor engineering
  5. Batch definition
  6. Process monitoring
  7. Analytics, bio-analytics
  8. Batch summary with yield, purity

Novel Recombinant Platforms (pl enquire):

  1. Novel promoters with special fermentation and induction performance
  2. Novel host / vector systems
  3. Novel inducers
  4. Novel expression vectors for integrative, and non-integrative expression
  5. Novel amplification systems (CHO)